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5-HT (5-Hydroxytryptamine) (Serotonin)

  • Serotonin (5-Hydroxytryptamine or 5-HT) is a monoamine neurotransmitter derived from the amino acid tryptophan.

  • It influences a wide range of physiological and behavioral processes.

5-HT (5-Hydroxytryptamine) (Serotonin)
Structure of 5-HT (5-Hydroxytryptamine) (Serotonin)

Synthesis:

  • From the amino acid tryptophan by tryptophan hydroxylase and subsequent decarboxylation.

Storage:

  • High concentration in enterochromaffin cells in the gut, platelets, and in the CNS as a neurotransmitter.

Metabolism:

  • Primarily by monoamine oxidase (MAO).

Functions of 5-HT:

  • Central Nervous System: Regulates mood, appetite, sleep, and cognition.

  • Gastrointestinal Tract: Controls intestinal movements and secretion.

  • Vascular System: Modulates vascular tone and platelet aggregation.

Pharmacological Effects of Serotonin

Here is a concise summary of those (actually histamine) effects:

  • Vasodilation of small vessels → lowers blood pressure

  • Increased capillary permeability → leads to edema and urticaria

  • Smooth muscle contraction (via H1) → causes bronchoconstriction

  • Stimulation of gastric acid secretion (via H2)

5-HT Receptors:

  • Serotonin acts through at least 14 different receptor subtypes, grouped into seven families (5-HT1 to 5-HT7). These receptors are involved in diverse physiological responses.

5-HT1 Receptors:

  • Subtypes: 5-HT1A, 1B, 1D, etc.

  • Functions: Inhibit adenylate cyclase activity, involved in vasoconstriction and neurotransmission.

5-HT2 Receptors:

  • Subtypes: 5-HT2A, 2B, 2C.

  • Functions: Mediate smooth muscle contraction, platelet aggregation, and various CNS effects.

5-HT3 Receptors:

  • Function: Ion channel receptors involved in emesis (vomiting) and neurotransmission.

5-HT4 to 5-HT7 Receptors:

  • Functions: Diverse roles including modulation of neurotransmission, cardiac function, and circadian rhythms.

5-HT Antagonists

5-HT3 Antagonists:

  • Purpose: Mainly used as antiemetics to prevent nausea and vomiting associated with chemotherapy, radiation therapy, and surgery.

  • Examples: Ondansetron, granisetron, palonosetron.

  • Mechanism: Block 5-HT3 receptors in the central nervous system and gastrointestinal tract to inhibit the vomiting reflex.

5-HT2 Antagonists:

  • Purpose: Used in the treatment of schizophrenia, depression, and migraine.

  • Examples: Olanzapine, risperidone (antipsychotics), cyproheptadine (antihistamine with 5-HT2 antagonism).

  • Mechanism: Block 5-HT2 receptors to modulate neurotransmission involved in mood and perception.

Other 5-HT Receptor Antagonists:

  • Purpose: Various applications depending on the specific receptor subtype targeted.

  • Examples: Trazodone (5-HT2 antagonism for depression and insomnia), pizotifen (migraine prophylaxis).

  • Below is a concise table summarizing selected serotonin (5-HT) receptor

Receptor

Location

Signaling Pathway

Pharmacological Actions

Example Agonist

Example Antagonist

5-HT1B/1D

Cerebral blood vessels, presynaptic nerves

Gi → ↓ cAMP

- Causes vasoconstriction in intracranial blood vessels- Inhibits nociceptive (pain) transmission

Sumatriptan (for migraine)

(No common clinical antagonist)

5-HT2A

Smooth muscle, platelets, CNS

Gq → ↑ IP3, DAG

- Vasoconstriction or vasodilation depending on location- Platelet aggregation- CNS effects (mood, perception)

(Not typically used clinically)

Ketanserin (experimental), Risperidone (antipsychotic)

5-HT2B

Heart (valves), stomach fundus

Gq → ↑ IP3, DAG

- Involved in cardiac valve fibrosis (when overstimulated)- Smooth muscle contraction in GI tract

(Not typically used clinically)

(No major selective antagonist)

5-HT2C

CNS (choroid plexus, hypothalamus)

Gq → ↑ IP3, DAG

- Appetite suppression- Mood regulation

Lorcaserin (for weight loss)

(No major selective antagonist)

5-HT3

Chemoreceptor trigger zone (CTZ), GI tract

Ligand-gated ion channel

- Emesis (vomiting reflex)- GI motility and secretion

(Not used clinically as agonist)

Ondansetron, Granisetron (antiemetics)

5-HT4

GI tract, CNS

Gs → ↑ cAMP

- Enhanced GI motility- Some CNS effects

Metoclopramide, Cisapride (prokinetic)

(No major selective antagonist)

Notes:

5-HT1 receptors (1B/1D) are key targets for migraine therapy (triptans).

5-HT2 receptors have diverse roles in the cardiovascular system, smooth muscle, and CNS. Antipsychotics often block 5-HT2A receptors.

5-HT3 is an ionotropic receptor crucial in the vomiting reflex. Antagonists like ondansetron are widely used to manage chemotherapy-induced nausea.

5-HT4 agonists are used as prokinetic agents to enhance GI motility.


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