HPLC is employed across various industries and research fields due to its robust separation capabilities and precision.
Quality Control:
Determining purity and content of pharmaceuticals.
Drug Development:
Identifying and quantifying drugs and their metabolites in biological matrices.
Impurity Profiling:
Identifying and quantifying impurities and degradation products in pharmaceutical compounds.
Formulation Studies:
Assessing the stability, dissolution, and other attributes of drug formulations.
Bioanalysis:
Analyzing biomolecules such as proteins and nucleic acids.
Environmental Analysis:
Detecting pollutants and contaminants in environmental samples.
Food and Beverage Industry:
Analyzing additives, preservatives, and contaminants.
Clinical Research:
Monitoring therapeutic drug levels and biomarkers.
Chemical Industry:
Monitoring reaction kinetics and product distributions.
Material Science:
Analyzing polymers and composites for volatile components.
Specialized Techniques:
Liquid Chromatography-Mass Spectrometry (LC-MS): Combines separation with structural identification.
Ultra High-Performance Liquid Chromatography (UHPLC): Enhances speed and resolution beyond traditional HPLC.