Advantages of HPLC
High Resolution: Effective separation of complex mixtures.
Versatility: Applicable to a wide range of compounds.
Precision: Reliable quantitative analysis.
Speed: Faster than traditional methods, especially with UHPLC.
Disadvantages of HPLC
Cost: High initial investment and maintenance.
Maintenance: Regular upkeep and column replacement needed.
Skill Requirement: Requires trained operators for optimal use.
Solvent Consumption: High solvent use can be costly and environmentally impactful.