Electrophoresis is a versatile technique with a wide array of applications.
Here are some of the key applications in brief:
1. DNA Sequencing:
Gel electrophoresis is used in Sanger sequencing methods to separate DNA fragments of different lengths.
2. Genotyping:
Electrophoresis helps determine the presence or absence of certain genes or specific DNA sequences in a sample.
3. Protein Analysis:
To determine protein sizes, charges, and purity. SDS-PAGE (a type of gel electrophoresis) is commonly used for protein separation based on size.
4. RNA Analysis:
To study RNA molecules, their sizes, and structures.
5. Forensic Analysis:
DNA fingerprinting in forensic science for identifying individuals or determining paternity.
6. Clinical Diagnostics:
Detection of certain diseases by identifying altered protein or DNA profiles.
7. Drug Testing:
Identifying specific compounds or metabolites in biological samples.
8. Research & Development:
Studying gene expression, protein interactions, and other molecular biology and biochemistry research areas.
9. Purification:
Preparative electrophoresis is used to isolate specific molecules from a mixture for further analysis or use.
10. Environmental Analysis:
Detecting pollutants or specific compounds in environmental samples.
