top of page
Search

Application of UV Vis spectroscopy

UV-Vis spectroscopy is widely used due to its simplicity, sensitivity, and versatility. Key applications include:

Spectrophotometric Titrations

Monitoring absorbance changes during titration to determine analyte concentrations.

  • Types:

    • Direct: Analyte absorbs light; absorbance changes with titrant addition (e.g., dye titration with a bleaching agent).

    • Indirect: Absorbance of an indicator changes during titration (e.g., acid-base titration with a pH indicator).

    • Back Titration: Excess titrant is measured after reacting with the analyte (e.g., calcium carbonate analysis).

    • Advantages: High sensitivity, selectivity, and automation.

    • Applications: Environmental analysis (pollutants), pharmaceuticals (active ingredients), food (additives).

  • Applications:

    • Environmental: Pollutant analysis (nitrates, phosphates).

    • Pharmaceuticals: Active ingredient quantification.

    • Food: Additive and colorant measurement.

Single Component Analysis

Determining one analyte's concentration using its unique absorbance.

  • Methods:

    • Direct Measurement: At λₘₐₓ using a calibration curve (e.g., caffeine in beverages).

    • Standard Addition: Accounting for matrix effects (e.g., vitamin C in juice).

    • Calibration Curve: Relating absorbance to concentration (e.g., cholesterol measurement).

    • Advantages: Simple and accurate for well-defined samples.

    • Limitations: Interference from other species and the need for a unique absorption peak.

    • Applications: Clinical diagnostics (hemoglobin), chemical manufacturing, research.

  • Applications:

    • Clinical: Hemoglobin measurement.

    • Manufacturing: Reactant monitoring.

    • Research: Isolated compound analysis.

Multi-Component Analysis of UV Vis spectroscopy

Determining concentrations of multiple species in a mixture.

  • Techniques:

    • Simultaneous Equations: Solve absorbance equations for components (e.g., drug mixtures).

    • Spectrum Subtraction: Isolates one component by subtracting another.

    • Derivative Spectroscopy: Resolves overlapping peaks.

    • Multivariate Calibration: Statistical methods (e.g., Partial Least Squares) for complex mixtures.

    • Advantages: Efficient, reduces sample volume.

    • Limitations: Complex analysis requiring calibration and software.

    • Applications: Pharmaceuticals (active ingredients), environmental monitoring (pollutants), food (colorants).

  • Applications:

    • Pharmaceuticals: Multi-drug assays.

    • Environmental: Detecting multiple pollutants.

    • Food: Mixture analysis of additives and preservatives.


Related Posts

See All

Deviations from Beer-Lambert Law

Situations where Beer-Lambert Law may not hold include: Chemical Deviations: Association/Dissociation : At high concentrations, molecules...

Attention!  We are facing some technical difficulties from google end because of which you may see empty blank spaces in between text. Please Ignore them, that does not mean incomplete or missing content. we are working on resolving the issue.  Keep Learning from world's best exam Notes!

bottom of page