Protein Purification:
Purification of monoclonal and polyclonal antibodies using Protein A or G.
Isolation of enzymes or recombinant proteins with affinity tags (e.g., His-tag, GST-tag).
Isolation of Biomolecules:
Separation of nucleic acids using immobilized complementary strands or aptamers.
Purification of carbohydrate-binding proteins or glycoproteins using lectin affinity chromatography.
Biomolecular Interaction Studies:
Studying binding kinetics and specificity between ligands and their targets.
Identifying unknown binding partners by capturing the protein of interest and then analyzing what co-elutes.