Capillary electrophoresis: Application, Advantages, Disadvantages & Limitations

Applications:

• Pharmaceuticals: Determining drug purity, analyzing metabolites.

• Biotechnology: Analyzing proteins, peptides, and nucleic acids.

• Forensics: DNA fingerprinting and drug analysis.

• Environmental: Detecting pollutants in water and soil.

Here is a colored diagram illustrating the applications of Capillary Electrophoresis

Advantages:

1.High Resolution:

• Capillaries can provide exceptional separation efficiency and resolution.

2. Speed:

• Faster runs due to high electric fields being applied without much heat generation.

3. Low Sample Requirement:

• Requires a very small amount of sample.

4. Automation:

• Automated sample injection and detection are possible.

Here is a colored diagram illustrating the advantages of Capillary Electrophoresis

Disadvantages:

1. Cost:

• Initial setup and equipment are relatively more expensive.

2. Maintenance:

• Capillaries can become clogged and might require regular maintenance.

3. Expertise:

• Higher skill level required for operation and troubleshooting.

Here is a colored diagram illustrating the disadvantages of Capillary Electrophoresis

Limitations:

• Challenges in method development.

• Sensitivity can be lower than some other methods, especially for non-UV absorbing compounds.

Here is a colored diagram illustrating the limitations of Capillary Electrophoresis: