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Classification and Mode of Action of Disinfectants

  • Disinfectants are chemical agents used to eliminate or reduce harmful microorganisms on surfaces and objects.

  • They are essential for controlling infections and maintaining hygiene in various settings, including healthcare, laboratories, and public spaces.

  • Disinfectants can be classified based on their chemical nature and their mode of action.

Classification by Chemical Nature of Disinfectants:

Classification by Disinfectants

1. Alcohols

  • Examples: Ethanol, Isopropanol

  • Mode of Action: Denature proteins, disrupt cell membranes.

  • Use: Commonly used for skin antiseptics and surface disinfection.

2.Aldehydes

  • Examples: Formaldehyde, Glutaraldehyde

  • Mode of Action: Cross-linking proteins and nucleic acids, leading to cell death.

  • Use: Sterilization of medical equipment and instruments.

3.Phenolics

  • Examples: Phenol, Cresols, Chlorhexidine

  • Mode of Action: Denature proteins and disrupt cell membranes.

  • Use: Disinfecting surfaces, particularly in hospitals and laboratories.

4.Halogens

  • Examples: Chlorine, Iodine, Hypochlorites (bleach)

  • Mode of Action: Oxidize cellular components, including proteins and nucleic acids.

  • Use: Water disinfection, surface cleaning, antiseptic solutions.

5. Oxidizing Agents

  • Examples: Hydrogen Peroxide, Peracetic Acid, Ozone

  • Mode of Action: Produce free radicals that damage proteins, DNA, and cell membranes.

  • Use: Sterilizing medical equipment, disinfecting surfaces, and water treatment.

6.Quaternary Ammonium Compounds (Quats)

  • Examples: Benzalkonium Chloride, Cetylpyridinium Chloride

  • Mode of Action: Disrupt cell membranes and denature proteins.

  • Use: Surface disinfectants, antiseptics, and in some sanitizers.

7.Heavy Metals

  • Examples: Silver Nitrate, Mercuric Chloride

  • Mode of Action: Bind to proteins and enzymes, inactivating them.

  • Use: Limited due to toxicity; used in some topical antiseptics and preservatives.

8.Biguanides

  • Examples: Chlorhexidine, Polyhexanide

  • Mode of Action: Disrupt cell membranes, leading to leakage of cellular contents.

  • Use: Skin antiseptics, disinfectants in medical settings.

9.Acids and Alkalis

  • Examples: Acetic Acid, Lactic Acid, Sodium Hydroxide

  • Mode of Action: Alter pH, denaturing proteins and disrupting cell membranes.

  • Use: Food industry, surface disinfection, and cleaning.

Classification by Spectrum of Activity:

Broad-Spectrum Disinfectants

  • Effective against bacteria, viruses, fungi, and spores.

  • Examples: Bleach (sodium hypochlorite), Glutaraldehyde

Intermediate-Spectrum Disinfectants

  • Effective against bacteria and enveloped viruses but not non-enveloped viruses or spores.

  • Examples: Quaternary ammonium compounds, Alcohols

Limited-Spectrum Disinfectants

  • Effective mainly against bacteria and some enveloped viruses.

  • Examples: Phenolics, some aldehydes.


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