The Lineweaver-Burk plot, also known as the double-reciprocal plot, is a graphical representation used to determine important kinetic parameters of an enzyme-catalyzed reaction, specifically the Michaelis constant (𝐾𝑚) and the maximum reaction velocity (𝑉max).
Lineweaver-Burk Equation
Description
The Lineweaver-Burk plot, or the double reciprocal plot, graphs 1/v (reciprocal of the reaction rate) against 1/[S] (reciprocal of the substrate concentration).
This transformation facilitates a linear relationship that simplifies the determination of Vmax and Km.
In this plot:
The y-intercept equals 1/Vmax.
The x-intercept is −1/−Km.
The slope represents Vmax/Km.
Advantages and Uses
The linear nature of the Lineweaver-Burk plot allows for a straightforward extraction of kinetic parameters. Additionally, this plot is particularly valuable in enzyme inhibition studies.
By comparing changes in the plot's slope, y-intercept, and x-intercept, one can deduce the type of inhibition (competitive, non-competitive, or uncompetitive) affecting the enzyme.