Nature of the Resin: Functional groups and crosslinking influence selectivity, capacity, and exchange rate.
pH of the Mobile Phase: Affects the charge state of ionizable compounds, influencing binding.
Ionic Strength: Higher ionic strength can promote elution through increased ion competition.
Temperature: Impacts exchange kinetics and resin capacity.
Particle Size of the Resin: Smaller particles offer better resolution but may increase back pressure.
Flow Rate: Must balance between sufficient contact time for exchange and process efficiency.
Concentration of Sample Ions: High concentrations can saturate the resin, reducing separation efficiency
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