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Instrumentation in Gas Chromatography

Instrumentation in Gas Chromatography
Instrumentation in Gas Chromatography

1) Sample Introduction System

  • Injection Port: Heated port for sample introduction and vaporization.

  • Auto-samplers: Automates sample introduction for precision and high throughput.

2) Mobile Phase (Carrier Gas) System

  • Carrier Gas Cylinder: Supplies inert gas (helium, hydrogen, or nitrogen).

  • Pressure Regulators and Flow Controllers: Maintain constant flow rate and desired pressure.

3) Columns

  • Packed Columns: Wider columns with solid stationary phase (used in older systems).

  • Capillary Columns: Narrow columns with stationary phase coated on walls, offering higher efficiency.

4) Oven

  • Provides temperature control for the column.

  • Operates at constant or programmed temperatures for improved separation.

5) Detectors

  • Flame Ionization Detector (FID): Measures ions from sample combustion.

  • Thermal Conductivity Detector (TCD): Detects changes in thermal conductivity.

  • Electron Capture Detector (ECD): Sensitive to halogenated compounds.

  • Mass Spectrometer (MS): Identifies compounds by mass-to-charge ratio.

6) Data Handling System

  • Computer interface for instrument control, data acquisition, and analysis.

  • Software aids in peak identification, integration, quantification, and reporting.

7) Additional Components

  • Split/Splitless Injector: Allows partial or full sample introduction.

  • Backflush System: Reverses flow to remove heavy or unwanted materials after analysis


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