Principle of IR spectroscopy:
Infrared (IR) spectroscopy involves the interaction of IR light with molecules, leading to vibrations within molecular bonds.
How it Works:
Absorption:
Molecules absorb specific IR frequencies, causing vibrational transitions in bonds (e.g., stretching or bending).
Spectrum Generation:
Remaining radiation passes to a detector, generating a spectrum of intensity vs. frequency, revealing absorbed regions indicative of chemical bonds.
Fingerprinting:
Each molecule has a unique IR absorption pattern, with bonds like C-H, N-H, and O-H showing distinct absorptions.
Pharmaceutical Applications:
Identification: Verifies the chemical composition, ensuring purity and consistency.
Quantitative Analysis: Measures the amount of a specific compound in a sample.
Formulation Analysis: Ensures correct ingredients and detects impurities in drug formulations.
Physical State: Identifies whether a drug is crystalline or amorphous.