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Methods of adjusting isotonicity

  • Methods of adjusting isotonicity of a solution is crucial in many medical and pharmaceutical applications to prevent cellular damage or discomfort upon administration.

  • The goal is to match the osmotic pressure of the solution to physiological fluids, such as blood or tears (~290 mOsmol/kg).

Methods of adjusting isotonicity
Methods of adjusting isotonicity

1) Class I Methods of adjusting isotonicity (Cryoscopic method):

  • Based on Freezing Point Depression:The formula used is:

    • Uses the formula:

ΔT = Kf * m

  • Where:

    • ΔT is the change in freezing point,

    • Kf is the cryoscopic constant,

    • m is the molality of the solute.

  • Adjust the molality to achieve the desired change in freezing point, which corresponds to isotonicity.

2) Class II Method (Osmotic Pressure Method):

  • Based on Osmotic Pressure:

    • Formula:

π = i M R * T

  • Where:

    • π is the osmotic pressure,

    • i is the van't Hoff factor (number of particles produced upon dissociation),

    • M is the molarity of the solute,

    • R is the ideal gas constant,

    • T is the temperature in Kelvin.

  • Adjust solute concentration to match the osmotic pressure of an isotonic solution.

3) White-Vincent Method:

  • Based on Isotonic Equivalents:

IE = (w / MW) * i

  • Where:

    • IE is the isotonic equivalent,

    • w is the weight of the solute in grams,

    • MW is the molecular weight of the solute,

    • i is the van't Hoff factor.

  • Calculate isotonic equivalents for all solutes and determine the amount of isotonicity-adjusting substance needed.

4) Sprowls Method:

  • Based on Equivalent Weights:

  • Formula:

Equivalent weight (EW)=M/100 × osmotic coefficient (φ)

Where:

  • EW is the equivalent weight,

  • M is the molecular weight of the solute,

  • φ is the osmotic coefficient.

Determine the total equivalent weight of solutes and adjust accordingly to achieve isotonicity.


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