Function
Breaks down penicillin, conferring resistance to β-lactam antibiotics.
Sources
Bacillus cereus
Staphylococcus aureus
Production Method
1. Isolation: Microorganisms isolated from soil, water, or animal waste.
2. Strain Selection: High-yield producers are selected.
3. Fermentation: Grown in a sterile medium with nutrients.
4. Harvesting: Enzyme extracted via centrifugation/filtration.
5. Purification: Using ammonium sulfate precipitation, ion-exchange, or affinity chromatography.
6. Stabilization: Adding stabilizers (e.g., glycerol, sucrose) to maintain activity.
7. Formulation: Prepared as a liquid or lyophilized powder.
