Literature Review:
Study existing delivery systems, challenges, and pharmacokinetics/dynamics of the drug.
Drug Selection:
Evaluate drug properties (molecular weight, solubility, stability) and therapeutic potential.
Understanding Barriers:
Analyze physiological and functional characteristics of ocular barriers and strategies to bypass them.
Initial Formulation Development:
Experiment with nanoparticles, liposomes, prodrugs, or implants.
In-Vitro Testing:
Assess drug release rates, stability, and cytotoxicity.
Ex-Vivo Permeation Studies:
Test drug permeation through excised corneal tissues to evaluate formulation effectiveness.
Safety Evaluations:
Screen for irritation or adverse reactions using in-vitro models.
Feedback and Iteration:
Refine formulations and delivery methods based on test results.
This systematic approach ensures the development of safe, effective, and innovative ocular drug delivery systems