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Nepheloturbidometry

  • Nepheloturbidometry is an instrumental technique used to quantitatively analyze substances in solution by measuring their light-scattering properties.

Principle:

  • When a light beam passes through a sample containing suspended particles (larger than the wavelength of light), the particles scatter light in all directions.

  • Nephelometry: Measures light scattered at an angle (typically 90°) to the incident beam.

  • Turbidimetry: Measures the decrease in transmitted light intensity due to scattering and absorption.

  • The intensity of scattered light is directly proportional to the concentration of suspended particles in the sample.

Procedure:

  1. A light source emits a beam through the sample.

  2. Suspended particles in the sample scatter the light.

  3. A detector, placed at an angle (commonly 90°) to the incident light, measures the scattered light intensity.

  4. The measured intensity is compared with standard solutions of known concentrations to determine the concentration of particles in the sample.

Applications:

  • Quantifying macromolecules such as proteins in solution.

  • Measuring turbidity in water samples for quality analysis.

Nepheloturbidometry is valuable for applications requiring precise quantification of suspended particles in various solutions.


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