Bioassay
Bioassays are analytical methods that use living organisms, cells, or biological systems to measure the concentration or potency of a substance, such as a hormone or drug.
They are essential in pharmacology for ensuring the efficacy and safety of pharmaceutical agents.
Principles of Bioassay
Biological Response: Bioassays rely on the measurable biological response elicited by a substance on a living system.
Standardization: A known standard is used to calibrate the assay, allowing for comparison and quantification.
Sensitivity and Specificity: The assay must be sensitive enough to detect low concentrations and specific to the substance of interest.
Reproducibility: Consistent results must be achievable across different experiments and conditions.
Dynamic Range: The assay should operate effectively within the concentration range relevant to the substance's biological activity.
Applications of Bioassay
Pharmaceutical Development: Determining the potency and dosage of drugs.
Quality Control: Ensuring batch-to-batch consistency in pharmaceutical manufacturing.
Clinical Diagnostics: Measuring hormone levels in patient samples for diagnosis and monitoring.
Environmental Testing: Assessing the presence and concentration of biological contaminants.