Production of Griseofulvin (Antifungal Antibiotic)

Microorganism Used

• Penicillium griseofulvum

Fermentation Process

1. Fermenter Type: Stirred-tank bioreactor

2. Fermentation Method: Submerged fermentation

3. Medium Composition: 

   • Carbon source: Glucose, malt extract

   • Nitrogen source: Peptone, yeast extract

   • Trace elements: Magnesium, zinc

4. Conditions: 

   • pH: 5.5-7.0

   • Temperature: 24-28°C

   • Aeration: Moderate oxygen supply

   • Duration: 7-10 days

5. Downstream Processing: 

   • Filtration to remove biomass

   • Extraction using organic solvents (ethyl acetate)

   • Crystallization and drying to obtain pure griseofulvin

Applications

• Used to treat fungal infections of skin, nails, and hair