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Quality Control Tests for Parenteral Products

Physical Tests:

  • Particulate Matter Test: Detects visible and sub-visible particles using light obscuration or microscopy.

  • Leakage Test: Ensures container integrity via dye ingress, vacuum, or pressure methods.

  • Volume/Fill Test: Verifies fill volume within pharmacopeial limits.

  • Clarity Test: Ensures solution is free from turbidity and visible particles.

Chemical Tests:

  • pH Measurement: Confirms pH within the specified range.

  • Drug Content and Assay: Verifies active ingredient concentration via HPLC or UV spectroscopy.

  • Stability Testing: Assesses chemical, physical, and microbiological stability under accelerated and real-time conditions.

Microbiological Tests: (Quality Control)

  • Sterility Test: Confirms absence of microorganisms via direct inoculation or membrane filtration.

  • Pyrogen Testing: Detects fever-inducing substances.

  • Rabbit Test: Measures temperature rise in rabbits.

  • LAL Test: Detects bacterial endotoxins.

  • Antimicrobial Effectiveness Test: Ensures preservative efficacy in multi-dose formulations.

  • Container-Closure Integrity Test: Validates seal sterility using vacuum decay or dye penetration.

  • Biological Tests: For vaccines and biologics, confirms efficacy and safety.


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