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UNIT 1 - Instrumental methods of analysis
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UV Visible spectroscopy
Introduction
Electronic transitions
Chromophores
Auxochromes
Spectral shifts
Solvent effect on absorption spectra
Beer and Lambert’s law
Derivation
Deviations
Instrumentation
Application: Spectrophotometric titrations, Single component and multi component analysis
Fluorimetry
Theory
Concepts of singlet, doublet and triplet electronic states
internal and external conversions
Factors affecting fluorescence
Quenching
Instrumentation
Working of Flourimetry
Applications
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