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UV-Visible Spectroscopy

Overview

  • UV-Visible spectroscopy is a technique used to measure the absorption of light in the ultraviolet (200-400 nm) and visible (400-700 nm) regions of the electromagnetic spectrum by a sample.

  • This method provides valuable information about the electronic structure of molecules, particularly the presence of chromophores and their environment. It is widely utilized in:

    • Quantitative analysis

    • Identifying compounds

    • Studying reaction kinetics

    • Determining molecular concentrations

UV-Visible Spectroscopy
UV-Visible Spectroscopy

Basic Principle of UV Visible spectroscopy

  • When a molecule absorbs light, its electrons are excited from a lower energy orbital to a higher energy orbital.

  • The energy difference between these orbitals corresponds to the energy of the absorbed photon, which is inversely proportional to its wavelength.

  • By measuring the absorbance at specific wavelengths, various properties of the sample can be inferred.


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