UV-Visible spectroscopy is a form of absorption spectroscopy that deals with the ultraviolet (UV) and visible regions of the electromagnetic spectrum.
It's a widely used analytical technique, especially in the field of pharmacy and chemistry.
Principle
UV-Visible spectroscopy operates based on the principle that certain molecules absorb UV or visible light at specific wavelengths.
When light passes through a sample, certain wavelengths are absorbed, resulting in an increase in the energy level of electrons.
This promotes them from a ground state to a higher energy or excited state.
The amount of light absorbed is related to the concentration of the absorbing species in the sample.
