Applications of Affinity Chromatography

Applications of Affinity Chromatography include purification of proteins, enzymes, and antibodies in biotechnology.

Applications of Affinity Chromatography

1. Protein Purification:

   • Purification of monoclonal and polyclonal antibodies using Protein A or G.
   • Isolation of enzymes or recombinant proteins with affinity tags (e.g., His-tag, GST-tag).

2. Isolation of Biomolecules:

   • Separation of nucleic acids using immobilized complementary strands or aptamers.
   • Purification of carbohydrate-binding proteins or glycoproteins using lectin affinity chromatography.

3. Biomolecular Interaction Studies:

   • Studying binding kinetics and specificity between ligands and their targets.
   • Identifying unknown binding partners by capturing the protein of interest and then analyzing what co-elutes.

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