Microorganisms Used in Production of Vitamin B12:
- Propionibacterium freudenreichii
- Pseudomonas denitrificans
- Bacillus megaterium
Fermentation Process:
- Fermenter Type: Aerated stirred-tank fermenter
- Fermentation Method: Submerged fermentation
- Medium Composition:
- Carbon source: Molasses, glucose
- Nitrogen source: Corn steep liquor, yeast extract
- Cobalt salts (essential for B12 biosynthesis)
- Conditions:
- pH: 6.0-7.5
- Temperature: 28-30°C
- Aeration: High oxygen supply
- Duration: 3-5 days
- Downstream Processing:
- Filtration to remove biomass
- Vitamin B12 extracted using solvents
- Purification by chromatography or precipitation
Advertisements
Applications:
- Used to treat pernicious anemia and in animal feed supplements
